Determination of Some Amino acids from Three Species of Plagiochasma by High Performance Thin Layer Chromatography (HPTLC)

The objective of the present study is to obtain the information about the presence or absence of eight amino acids and to determine their content in three species of Plagiochasma by HPTLC. Out of eight presently studied amino acids, six are essential amino acids (methionine, threonine, valine, leucine, histidine and isoleucine) and two are non- essential amino acids (alanine and glycine). Among the three species of Plagiochasma, P. appendiculatum shows the highest content of all amino acids except methionine, ranging between 0.138 mg/g dw (of alanine) and 0.644 mg/g dw (of glycine). In P. articulatum histidine content (0.177 mg/g dw) is more than the rest of the amino acids. In P. intermedium glycine content (0.151 mg/g dw) is highest among the other amino acids. P. articulatum and P. intermedium show all the eight amino acids, whereas P. appendiculatum does not contain methionine.