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A SINGLE LIVING BACTERIUM'S REFRACTIVE INDEX MEASUREMENT BY USING OPTOFLUIDIC IMMERSION REFRACTOMETRY

Yang Liu, L. K. Chin, W. Ser, T. C. Ayi, Wei-De Ho, P. H. Yap, Yamin Leprince‐Wang, Tarik Bourouina

Open publisher page 3 citations

Abstract

This paper presents a biophysical method to characterize single bacterium in water by using an on-chip immersion refractometer. The working principle is based on immersion refractometry, whereby the refractive index of a single bacterium is measured using the difference between the various refractive indices. The preliminary results show that E. coli is larger in term of width and has a lower refractive index value as compared to bacillus subtilis. The database for protozoa will be expanded by measuring different kinds of bacteria using the on-chip immersion refractometer.

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What this paper is about

This paper presents a biophysical method to characterize single bacterium in water by using an on-chip immersion refractometer. The working principle is based on immersion refractometry, whereby the refractive index of a single bacterium is measured using the difference between the various refractive indices. The preliminary results show that E. coli is larger in term of width and has a lower refractive index value as compared to bacillus subtilis. The database for protozoa will be expanded by measuring different kinds of bacteria using the on-chip immersion refractometer.

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OpenAlex reports 3 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

This paper presents a biophysical method to characterize single bacterium in water by using an on-chip immersion refractometer. The working principle is based on immersion refractometry, whereby the refractive index of a single bacterium is measured using the difference between the various refractive indices. The preliminary results show that E. coli is larger in term of width and has a lower refractive index value as compared to bacillus subtilis. The database for protozoa will be expanded by measuring different kinds of bacteria using the on-chip immersion refractometer.

Key concepts: Refractometry, Refractometer, Refractive index, Bacillus subtilis, Immersion (mathematics), Optics, Materials science, Optofluidics

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