Isolation and Quantification of Aflatoxin from Aspergillus Flavus Infected Rice

Isolation of Apergillus flavus from groundnut and production of aflatoxin on rice. a method has been developed for the production of af latoxin by growing Aspergillus flavus strain AJ on the solid substrate rice. Optimal yields, more tha n 1mg of aflatoxin B1 per gram of starting material, were obtained in 5 days at 28C 0 .A crude product containing aflatoxin was isolated by HPLC (High Performance Liquid Chromatography). The cruded product consists of 55% aflatoxin in the following ratio:B1-B2-G1- G2,100:0.15:0.22:0.02.Aflatoxin B1 was separated from all the impurities and from the other aflatoxins by chromatograph on silica gel wi th 1% ethyl alcohol in chloroform.