Comparison Of The Effects Of Monochloramine And Glutaraldehyde (Biocides) Against Biofilm Microorganisms In Produced Water

Abstract – The comparison of the effects of oxidizing biocide (monochloramine) and non-oxidizing biocide (glutaraldehyde) against biofilm microorganisms in produced water system have been investigated. Batch process was used for the investigation of biofilm attachment on polyvinylchloride slides with the aid of chocolate agar (enriched medium), in produced water. The effects of the biocides on biofilm mass were assessed by comparing the biofilm mass of the control samples (without the addition of biocides) to the biofilm mass of the test samples (after the addition of biocides). The organic constituents of the biofilm on each slide were evaluated as spectrophotometric analysis of protein and polysaccharide contents using modified Lowry method and phenol-sulphuric acid method, respectively. Monochloramine proved to be more effective in reducing the biofilm mass than glutaraldehyde. Considering reduction in total protein contents and a 10 day old biofilm, monochloramine caused a 62.9 % reduction while glutaraldehyde caused a 59.1 % reduction.