1965•Unpublished venueRequires access
Location andRegeneration ofEnterovirus Receptors ofHeLaCells
Langbord VirusLaboratory
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Abstract
ZAJAC, IHOR(Hahnemann Medical College, Philadelphia, Pa.), AND RICHARDL. CROWELL.Location andregeneration ofenterovirus receptors ofHeLacells. J.Bacteriol. 89:1097-1100. 1965.-Treatment ofliveHeLacells withchymotrypsin ortrypsin completely inactivated theviral receptors forcoxsackievirus B3andpoliovirus Ti, respectively. Enzyme-treated cells regained their enterovirus receptor activity after incubation inculture medium.Theexistence ofintracellular receptors forvirus could notbedemonstrated whenliveHeLacells were treated withenzyme prior todisruptionandfractionation. Theseresults suggested thatreceptors forenteroviruses are limited tothecell surface. Itwasreported recently (Zajac andCrowell, 1965) thatproteolytic enzymes willselectively inactivate enteroviral receptors on liveHeLa cells without affecting cell viability. Thisfinding hasmadeitpossible toreinvestigate theintracellular distribution of enterovirus receptors (Holland andMcLaren, 1961;Philipson and Bengtsson, 1962) andtostudy receptor regeneration. Thiscommunication provides evidence that enteroviral receptors arelimited to thecell surface, and thatenteroviral receptors are regenerated inamannersimilar tothatfound for receptors formyxoviruses (Fazekas deSt.Groth, 1948;Stone, 1948a, b;Baluda, 1958; Marcus, 1959) andforEMC virus (Kodza andJungeblut, 1958).