Comparison of kinetin and 6-banzyl amino purine effect on in vitro microtuberization of two cultivars of potato (Solanum tuberosum L.).
Elyas Aryakia, Yousef Hamidoghli
Abstract
Elyas Aryakia, Yousef Hamidoghli
Abstract
In the present study, in vitro microtuberization of two potato cultivars, Arinda and was investigated. The basal medium consisted of the Murashige and Skoog medium supplemented with 80 gl 1 sucrose and 8 gl agar. The effects of 4 concentrations (0.25, 0.50, 0.75 and 1 mg.l ) of kinetin and 6- banzyl 11 amino purine (BAP) on physical characteristics such as number, size and weight of potato microtuber were investigated. Sixty days after culture, microtubers were harvested and data analyzed. Results showed that the highest effect on microtuber number especially in cv. Diamant with an average number 1.49 per single node explants, was obtained due to kinetin role in tuber initiation. BAP especially in high concentrations (0.75 and 1 mg.l ) have an incremental effect on weight and size of microtuber. Since a large size of microtuber is suitable 1
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In the present study, in vitro microtuberization of two potato cultivars, Arinda and was investigated. The basal medium consisted of the Murashige and Skoog medium supplemented with 80 gl 1 sucrose and 8 gl agar. The effects of 4 concentrations (0.25, 0.50, 0.75 and 1 mg.l ) of kinetin and 6- banzyl 11 amino purine (BAP) on physical characteristics such as number, size and weight of potato microtuber were investigated. Sixty days after culture, microtubers were harvested and data analyzed. Results showed that the highest effect on microtuber number especially in cv. Diamant with an average number 1.49 per single node explants, was obtained due to kinetin role in tuber initiation. BAP especially in high concentrations (0.75 and 1 mg.l ) have an incremental effect on weight and size of microtuber. Since a large size of microtuber is suitable 1
Key concepts: Kinetin, Solanum tuberosum, Explant culture, Cultivar, Agar, Horticulture, Sucrose, Chemistry