Analysis based on AFLP markers of the genetic variations and their relationships for pummelo cultivars grown in the central region of Thailand

Using 15 selected AFLP primers, this investigation looks at the genetic diversity and relationships among pummelo (Citrus maxima (Burm.) Merrill) cultivars grown in the central region of Thailand, resulting in amplification of 133 reproducible polymorphic fragment products out of 295 band score. The percentages of polymorphic markers for AFLP ranged from 33.33% for E-CGT/M-TGA and E-AAG/M-AGA to 62.50% for E-TAC/M-TCG. The phylogenetic tree dendrogram showed that the 97 leaf samples from 15 pummelo cultivars grown in central region of Thailand could be classified into two groups. The first group consisted of two sub-groups. The first sub-group consisted of Khaopuang, Takoi, Toolkaol, and Khaojook cultivar. The second one consisted of Khaopan, Khaohom, Khaonamphueng, Khaoyai, Phaiseethong, Khaotanggwa, Kkewmorakot, and seedless cultivar. The second group consisted of Thongdee, Bangkhunnon and Tabtim cultivar. Moreover, the results indicate that the samples collected from the same cultivar in different locations were for the main part genetically similar.