Studies on Effect of Nutrient Media for Clonal Propagation of Superior Phenotypes of Dalbergia sissoo Roxb. through Tissue Culture

Micropropagation studies were undertaken using nodal explants collected from two ramets (clone No. 36) belonging to 60-year-old superior tree of Dalbergia sissoo. Two nutrient media viz. MS and B5 were used to find out the suitability of the medium. Bud break was achieved in both of the media within 6-8 days under different media combinations supplemented with BAP (0.10‐1.0 mg/l) alone as well as in combinations with IAA or NAA (0.10 to 0.50 mg/l). Maximum percentage of bud break (100%) was achieved in both of the media. Maximum number of shoots per explant (8.04) was observed in the MS medium supplemented with 1.0 mg/l BAP + 0.25 mg/l NAA. Out of the two nutrient media tried MS medium was found to be the best, which gave high rate of shoot proliferation as compared to B5 medium. Maximum number of roots per plantlet (4.47) was observed in 1 /2 MS supplemented with (1.0 mg/l) IBA within 18 days. Plantlets so produced were acclimatized and transplanted to pots and 70% survival was achieved.