Production and optimization of cellulase enzyme by Pseudomonas aeruginosa MTCC 4643 using sawdust as a substrate

The main objective of the study is to explore easier and cost effective method to produce the cellulase enzyme by using sawdust as a substrate, which is an industrial waste. In the present investigation, different cultural conditions (Concentration of sawdust, pH, Temperature and Inoculum size) were examined to assess their effect for optimum cellulase production using Pseudomonas aeruginosa MTCC 4643. Pretreated sawdust produced highest cellulase activity (12.08 U/g)at 5% concentration. Optimum pH and temperature for cellulase production was observed 6.0 and 30 0 C respectively.