RESEARCH ARTICLE QUANTIFICATION OF TOTAL PHENOL, FLAVONOID CONTENTS AND ANTIOXIDANT ACTIVITY OF SOME SELECTED INDIAN MEDICINAL PLANTS

The study was conducted to investigate the phenol, flavonoid content and antioxidant activity in some selected, traditionally used Indian medicinal plants in ethyl acetate, and screened for their antioxidant and free radical scavenging properties using Ascorbic acid and α-tocopherol as standard antioxidants. Antioxidant was determined using spectrophotometric methods. Antioxidant activities of extracts were expressed as percentage of DPPH radicals inhibition and IC50 values. The total phenolic content ranged from 35.25 to 224.49 mg/100g of dry weight of extract, expressed as Gallic acid equivalents.The total Flavonoids concentration varied from 6.86 to 126.24 mg /100g of dry weight expressed as rutin equivalents. Results suggested a good potential of phenolic and flavonoid compounds and antioxidant activity in Strychnosnux-vomica (IC50value42.60),SalaciaOblonga(IC50 value 42.94) Indigofera inctoria (IC50 value 40.64) Melothria maderaspatana(IC50 value 38.21 ) and Enicostemma littorale(IC50 value 43.76) showed better activity than remaining medicinal plants.