2008•Unpublished venueRequires access
Loss of Heterozygosity for Chromosome 18q and Microsatellite Instability Are Highly Consistent Across the Region of the DCC and SMAD4 Genes in Colorectal Carcinomas and Adenomas
Peter Zauber, Marlene Sabbath‐Solitare, Stephen Marotta, D. Timothy Bishop
Open publisher page 3 citations
Abstract
Loss of heterozygosity (LOH) of chromosome 18q is clearly associated with colorectal carcinoma. The gene DCC (Deleted in Colorectal Cancer) is a putative tumor suppressor gene within the 18q21.2 region, but the importance of DCC has been questioned. A nearby gene called SMAD4 has been suggested as the critical tumor suppressor gene in this region. We evaluated a series of 110 colorectal carcinomas and adenomas for LOH of the DCC and SMAD4 genes to clarify the involvement of this DNA segment of chromosome 18q in colorectal carcinogenesis. We utilized 2 markers for each gene for all lesions and 5 additional markers for 23 lesions with inconsistent results, using standard PCR techniques. The benign and malignant portions of 50 in situ carcinomas were studied independently. Similar molecular results for