Study of Pollen Sterility in Clusterbean ( (L.) Taub. ) Through Mutagenesis Cyamopsis Tetragonoloba

The Cluster bean ( Cyamopsis tetragonoloba (L.) Taub. ) (2n=14) belongs to family Fabaceae. The importance of legume has been highly appreciated as a source of guar gum. Studies on pollen sterility can help to produce haploid plants for hybridization. The guar seeds have been used in Indian medicine as a laxative and a digestive tonic. It may be useful in prediabetic conditions and the early stages of late onset diabetes. Guar gum is also believed to lower cholesterol. The cultivar of cluster bean 'P.N.B' were treated with chemical mutagen Ethyl Methane Sulphonate (EMS) at the concentration of 4mM, 7mM, 9mM, 10mM, and physical mutagen gamma rays at the dosage of 100Gy, 200Gy, 300Gy, 400Gy, and also with combination of both (Gamma rays and EMS) like 100Gy + 10mM, 200Gy + 9mM, 300Gy + 7mM, 400Gy + 4mM. In M1 generation pollen sterility was studied. In control it was 9.25%. The maximum sterility was seen at 400Gy dosage. Of the two mutagens gamma rays revealed the maximum sterility than the EMS concentrations. Combination of both mutagens show low pollen sterility as compared to individual treatments.