Application to Dissolution studies and HPLC Assay Method for Determination of Diacerein and Aceclofenac in Tablet Dosage Form

2 Abstract: The proposed method is precise, accurate, selective and rapid for the simultaneous determination of Diacerein and Aceclofenac. A simple, rapid and selective HPLC method has been developed for quantitation of Diacerein and Aceclofenac from bulk drug and pharmaceutical formulations using a mobile phase consisting mixture of methanol and water (80:20 v/v) at the flow rate of 1mL/min. An ODS C18 RP-Column (Intersile 4.6 mm x 25 cm, 10 µm) column was used as stationary phase. The retention time of Diacerein and Aceclofenac were 4.6 min. and 7.3 min. respectively. Linearity was obtained in the concentration range of 5-35µg/ml for Diacerein, 5-35 µg/ml for Aceclofenac. Gatifloxacin was used as an internal standard. The method was statistically validated and RSD was found to be less than 2% indicating high degree of accuracy and precision of the proposed HPLC method. Due to its simplicity, rapidness, high precision and accuracy, the proposed HPLC method may be used for determining Diacerein and Aceclofenac in bulk drug samples or in pharmaceutical dosage form.