Addendum: qPCR of DNA Isolated with CTAB, DNeasy®, and Synergy™ Methods A follow up to the "Evaluation of the Synergy™ Rapid Plant DNA Isolation Chemistry" study.

A recent study by OPS Diagnostics examined and compared several methods of isolating DNA from plants (link). Parameters examined were DNA yield, purity (as measured by 260/280 and 260/230 ratios), and whether the DNA would amplify by standard PCR. The study concluded that the Synergy™ Plant DNA Extraction Kit is equal to or out performs a traditional CTAB method and DNeasy® kit for DNA isolation from several plants tested, including rye, rice, corn, sorghum, soybean, wheat, and rape seed. This addendum supplements the previous study by examining plant DNA isolated by the same methods and its subsequent analysis by qPCR. The objective is to assess the relative effect of each DNA isolation method on quantitation. A standard CTAB method and two commercial DNA isolation kits (DNeasy® Plant DNA Isolation Kit and Synergy™ Plant DNA Extraction Kit) were used to isolate DNA from grape, rice, and sorghum (link). Both the CTAB and DNeasy® methods yield DNA in TE buffer following alcohol precipitation. The Synergy™ Plant DNA Extraction Kit yielded two sample types, namely lysate from the initial purification step and DNA resuspended in TE buffer following alcohol precipitation.