2010•Unpublished venueRequires access

Yields of different plasmid DNA from E. coli cultures using the QuickPick TM Plasmid DNA kit

Sanna Pietilä, Pekka T. Mattsson

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Abstract

The QuickPick Plasmid DNA purification kit provides a fast and simple means of isolating plasmid DNA from bacterial cultures. Plasmid yields are dependent on the type of plasmid used as well as growth conditions and other factors. The purification technique does not require any organic solvents and eliminates the need for repeated centrifugation, vacuum filtration or column separation. The purified plasmid DNA is of high quality, suitable for downstream applications such as restriction enzyme digestion, ligation, DNA sequencing, E. coli transformation, PCR and cloning.

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What this paper is about

The QuickPick Plasmid DNA purification kit provides a fast and simple means of isolating plasmid DNA from bacterial cultures. Plasmid yields are dependent on the type of plasmid used as well as growth conditions and other factors. The purification technique does not require any organic solvents and eliminates the need for repeated centrifugation, vacuum filtration or column separation. The purified plasmid DNA is of high quality, suitable for downstream applications such as restriction enzyme digestion, ligation, DNA sequencing, E. coli transformation, PCR and cloning.

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Available abstract

The QuickPick Plasmid DNA purification kit provides a fast and simple means of isolating plasmid DNA from bacterial cultures. Plasmid yields are dependent on the type of plasmid used as well as growth conditions and other factors. The purification technique does not require any organic solvents and eliminates the need for repeated centrifugation, vacuum filtration or column separation. The purified plasmid DNA is of high quality, suitable for downstream applications such as restriction enzyme digestion, ligation, DNA sequencing, E. coli transformation, PCR and cloning.

Key concepts: Plasmid, Plasmid preparation, DNA, Restriction enzyme, Transformation (genetics), In vitro recombination, Biology, Molecular biology

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