Yields of different plasmid DNA from E. coli cultures using the QuickPick TM Plasmid DNA kit
Sanna Pietilä, Pekka T. Mattsson
Abstract
Sanna Pietilä, Pekka T. Mattsson
Abstract
The QuickPick Plasmid DNA purification kit provides a fast and simple means of isolating plasmid DNA from bacterial cultures. Plasmid yields are dependent on the type of plasmid used as well as growth conditions and other factors. The purification technique does not require any organic solvents and eliminates the need for repeated centrifugation, vacuum filtration or column separation. The purified plasmid DNA is of high quality, suitable for downstream applications such as restriction enzyme digestion, ligation, DNA sequencing, E. coli transformation, PCR and cloning.
A significance statement is not available in the OpenAlex record.
A contribution statement is not available in the OpenAlex record.
Method details are not available in the OpenAlex metadata.
Findings are not separately available in the OpenAlex metadata.
Limitations are not available in the OpenAlex metadata.
Application details are not available in the OpenAlex metadata.
The QuickPick Plasmid DNA purification kit provides a fast and simple means of isolating plasmid DNA from bacterial cultures. Plasmid yields are dependent on the type of plasmid used as well as growth conditions and other factors. The purification technique does not require any organic solvents and eliminates the need for repeated centrifugation, vacuum filtration or column separation. The purified plasmid DNA is of high quality, suitable for downstream applications such as restriction enzyme digestion, ligation, DNA sequencing, E. coli transformation, PCR and cloning.
Key concepts: Plasmid, Plasmid preparation, DNA, Restriction enzyme, Transformation (genetics), In vitro recombination, Biology, Molecular biology