ScholarLens Back to search
2013•Unpublished venueRequires access

A REVIEW ON BIOANALYTICAL METHOD DEVELOPMENT AND VALIDATION BY USING LC-MS/MS

S Murugan, P. Sirisha, K Chandrakala

Open publisher page 16 citations

Abstract

Development and validation of bioanalytical method is important to understand the pharmacokinetics of any drug and/or its metabolites. Liquid chromatography-mass spectrometry (LC-MS/MS) is a technique that uses liquid chromatography (or HPLC) with the mass spectrometry. (LC-MS/MS) is commonly used in laboratories for the qualitative and quantitative analysis of drug substances, drug products and biological samples. This article reviews various extraction techniques like liquid–liquid extraction, solid phase extraction and protein precipitation which play important role in sample preparation and detection by LC-MS/MS. Bioanalytical method validation includes all of the procedures that demonstrate that a particular method used for quantitative measurement of analytes in a given biological matrix, such as blood, plasma, serum, or urine is reliable and reproducible for the intended use.

About this research paper

What this paper is about

Development and validation of bioanalytical method is important to understand the pharmacokinetics of any drug and/or its metabolites. Liquid chromatography-mass spectrometry (LC-MS/MS) is a technique that uses liquid chromatography (or HPLC) with the mass spectrometry. (LC-MS/MS) is commonly used in laboratories for the qualitative and quantitative analysis of drug substances, drug products and biological samples. This article reviews various extraction techniques like liquid–liquid extraction, solid phase extraction and protein precipitation which play important role in sample preparation and detection by LC-MS/MS. Bioanalytical method validation includes all of the procedures that demonstrate that a particular method used for quantitative measurement of analytes in a given biological matrix, such as blood, plasma, serum, or urine is reliable and reproducible for the intended use.

Why it matters

OpenAlex reports 16 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Development and validation of bioanalytical method is important to understand the pharmacokinetics of any drug and/or its metabolites. Liquid chromatography-mass spectrometry (LC-MS/MS) is a technique that uses liquid chromatography (or HPLC) with the mass spectrometry. (LC-MS/MS) is commonly used in laboratories for the qualitative and quantitative analysis of drug substances, drug products and biological samples. This article reviews various extraction techniques like liquid–liquid extraction, solid phase extraction and protein precipitation which play important role in sample preparation and detection by LC-MS/MS. Bioanalytical method validation includes all of the procedures that demonstrate that a particular method used for quantitative measurement of analytes in a given biological matrix, such as blood, plasma, serum, or urine is reliable and reproducible for the intended use.

Key concepts: Bioanalysis, Chromatography, Protein precipitation, Analyte, Liquid chromatography–mass spectrometry, Sample preparation, Chemistry, Mass spectrometry

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
A REVIEW ON BIOANALYTICAL METHOD DEVELOPMENT AND VALIDATION BY USING LC-MS/MS — Research Paper | ScholarLens