2014•Unpublished venueRequires access

Optimization and production of Polyhydroxybutarate(PHB) by Bacillus subtilis G1S1from soil

K.R. Shah, H. D. Patel

Abstract

A B S T R A C T Polyhydroxybutyrate (PHB), a lipid-like polymer of 3-hydroxybutyrate, is a representative member of polyhydroxyalkanoates (PHAs) formed in many bacteria.12 bacterial isolates were found to be the most promising PHA accumulating bacteria among 15 isolates screened from soil. Screening for PHA was done by Sudan black staining. Bacillus subtilis G1S1 shows PHA maximum production under the different carbon and nitrogen condition. PHA extraction was carried out by chloroform digestion method. Biochemical and 16s rRNA analysis showed that PHA producing bacteria belong to Bacillus subtilis G1S1 genera with Maximum production of PHA was analyzed by U.V spectrophotometer and finally it was characterized by FTIR spectroscopy.

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Key concepts: Polyhydroxyalkanoates, Bacillus subtilis, Polyhydroxybutyrate, Bacteria, Bacillus (shape), Sudan Black B, 16S ribosomal RNA, Chemistry