Root Associated Bacteria from the Local Variety of Rice GJ-17 Antagonized the Growth of Magnaporthe grisea
Yachana Jhaa, NB Patel
Abstract
Yachana Jhaa, NB Patel
Abstract
Root associated bacteria were isolated from the rice field of Gujarat by using nitrogen free selective medium. The biochemical tests and molecular analysis were preformed for the identification of isolates. Then the sequences obtained were analyzed by using bioinformatics software and the cultures were identified as Pseudomonas pseudoalcaligenes, Bacillus pumilus and Stenotrophomonas maltophilia. Then these isolates were in-vitro inoculated to paddy plant to study the effect on plant growth promotion and resistance against pathogen. Results obtained during greenhouse and field assay showed antagonistic effect of these isolates against Magnaporthe grisea and isolates also enhance other growth parameters.
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Root associated bacteria were isolated from the rice field of Gujarat by using nitrogen free selective medium. The biochemical tests and molecular analysis were preformed for the identification of isolates. Then the sequences obtained were analyzed by using bioinformatics software and the cultures were identified as Pseudomonas pseudoalcaligenes, Bacillus pumilus and Stenotrophomonas maltophilia. Then these isolates were in-vitro inoculated to paddy plant to study the effect on plant growth promotion and resistance against pathogen. Results obtained during greenhouse and field assay showed antagonistic effect of these isolates against Magnaporthe grisea and isolates also enhance other growth parameters.
Key concepts: Magnaporthe grisea, Biology, Bacillus pumilus, Stenotrophomonas, Bacteria, Inoculation, Microbiology, Stenotrophomonas maltophilia