Antimicrobial Activity of the Masticatory Cola acuminata Nut (Gooro)

The aim of this study was to investigate the possible inhibitory activity of Cola acuminata seed (Petroleum ether, methanolic and aqueous) extracts against two standard Gram positive (Bacillus subtilis and Staphylococcus aureus), three Gram negative (Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumonae) bacteria and one fungus (Candida albicans). Petroleum ether, methanolic and aqueous extracts of Cola acuminata nuts at concentrations of 10, 12.5, 25 and 50% were screened for antimicrobial activity against two standard Gram positive (Bacillus subtilis and Staphylococcus aureus), three Gram negative (E. coli, Pseudomonas aeruginosa and Klebsiella pneumoneae) bacteria and one fungus (Candida albicans). No inhibitory activity was detected using all concentrations of petroleum ether extract for all tested microorganisms. Wide range of activity was exhibited by both aqueous and methanol extract. However, the former highest activity was detected against Staphylococcus aureus at the two concentration 25 and 50% (20 and 24 mm) while the latter was found against Candida albicans at the concentration of 25% (20 mm).