Quantitative estimation of negundoside in Nirgundi taila by HPLC

By optimizing the extraction, separation and analytical conditions, a reliable, rapid, simple and accurate liquid chromatography method with UV detection was developed for the quantitative determination of negundoside in an ayurvedic preparation Nirgundi taila. The estimation of negundoside was achieved on an reversed phase C 18 column (250 mm X 4.6 mm ID, 5 µm particle size), with isocratic elution using a mixture of acetonitrile-methanol (70: 30, v/v) at a flow rate of 1 mL min -1 with UV detection at 254 nm, the sample injection volume was 10µL. The calibration curve was linear with correlation coefficients of 0.9996 for negundoside. The % Relative standard deviation (%RSD) value was less than 2% in the concentration range of 10-100 µg mL-1. Intra-day assay and inter-day assay precision of the analyte was less than 2%, and the average recovery rate obtained was in the range of 98-102% with %RSD below 2%. This method can provide a scientific and technical platform to the product manufacturers for setting up a quality control standard as well as to the public for quality and safety assurance of the proprietary ayurvedic formulations.