16S rDNA analysis for characterization of Pseudomonas sp. strain MF30 isolated from Rumex acetocella roots in northern Sweden
Idress Hamad Attitalla, Ali A. Bataw, J. Borowicz, Sture Brishammar
Abstract
Idress Hamad Attitalla, Ali A. Bataw, J. Borowicz, Sture Brishammar
Abstract
A bacterial strain obtained from the northern part of Sweden previously classified as Pseudomonas veronii based on biochemical and physioloical tests. In this study, phylogenetic tree was constructed using a nearly complete sequence within the 16S rDNA gene. The strain of Pseudomonas sp. subdivided into two rather distinctly related groups, neither of which is very close to the group within the Pseudomonas fluorescens cluster. Although the phylogenetic analysis is not conclusive, it is consistent with other observations, especially the capacities of the this strain as a biocontrol agent. Taken all together, the results suggest that the MF30 strain should be classifed as another Pseudomonas species, either Pseudomonas antarctica or P. meridina.
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A bacterial strain obtained from the northern part of Sweden previously classified as Pseudomonas veronii based on biochemical and physioloical tests. In this study, phylogenetic tree was constructed using a nearly complete sequence within the 16S rDNA gene. The strain of Pseudomonas sp. subdivided into two rather distinctly related groups, neither of which is very close to the group within the Pseudomonas fluorescens cluster. Although the phylogenetic analysis is not conclusive, it is consistent with other observations, especially the capacities of the this strain as a biocontrol agent. Taken all together, the results suggest that the MF30 strain should be classifed as another Pseudomonas species, either Pseudomonas antarctica or P. meridina.
Key concepts: Pseudomonas, 16S ribosomal RNA, Pseudomonas fluorescens, Phylogenetic tree, Strain (injury), Biology, Microbiology, Sequence analysis