Participation of Large Ca2+ Activated Potassium Channel in Antinociceptive Activity of Chalcone Derivative (3-(2, 5-dimethoxyphenyl)-1-(5-methylfuran-2-yl) prop-2-en-1-one) DMPF-1 Action in Mice Model

The role of potassium channels in nociceptive activity \nwas proposed in the past decade. Various type of potassium channel has been found to exert different action in propogation of action potential in nervous system. As DMPF-1, a chalcone derivative possesses antinociceptive properties. The mechanism of its action has been carried out to verify the pathway involved. The present study addressed the role of potassium channel in the contribution of the antinociceptive action of DMPF-1. The involvement of potassium channel was evaluated using acetic acid-induced abdominal writhing test.The animals were pretreated with charybdotoxin (large Ca2+ activated potassium channel blocker)(0.04mg/kg, i.p) or apamin (small Ca2+ activated potassium channel blocker)(0.02mg/kg, i.p.) 15 \nminutes before administration of DMPF-1. It was demonstrated that the challenge of DMPF-1 treated group with charybdotoxin has reversed the antinociceptive activity of this novel chalcone, which indicates the possible participation of large Ca2+ activated potassium \nchannel in antinociceptive effect cause by DMPF-1 but not through small Ca2+ activated potassium channel.