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2010•ROMANIAN BIOTECHNOLOGICAL LETTERSRequires access

Genetic diversity of some Romanian grapevine cultivars as revealed by microsatellite markers.

A. Anghel, S. Zamfirescu, Carmen Dragomir, D. Nadolu, E. Sogorescu, F. Busuricu

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Abstract

The PCR-based DNA microsatellite analysis has been applied to define genetic relationships among eleven Romanian grapevine varieties. 87 alleles with a mean number of 7.90 alleles per locus were detected in the set of cultivars analyzed. The total number of alleles per locus varied between 4 (in VVMD24 and VVIQ52) and 10 (in VMC8G9, UDV125 and VMC5G6.1). The expected heterozygosity (gene diversity) ranged from 0.63 at locus VVMD24 to 0.92 at locus VVMD28. The observed heterozygosity varied between 0.63 at locus VVMD24 to 1 at locus VVMD28 and was higher than the expected one at 4 out of 11 loci. Microsatellite analysis proved to be useful for the genetic characterization of Romanian grapevines, allowing precise identification and good discrimination of all tested cultivars.

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What this paper is about

The PCR-based DNA microsatellite analysis has been applied to define genetic relationships among eleven Romanian grapevine varieties. 87 alleles with a mean number of 7.90 alleles per locus were detected in the set of cultivars analyzed. The total number of alleles per locus varied between 4 (in VVMD24 and VVIQ52) and 10 (in VMC8G9, UDV125 and VMC5G6.1). The expected heterozygosity (gene diversity) ranged from 0.63 at locus VVMD24 to 0.92 at locus VVMD28. The observed heterozygosity varied between 0.63 at locus VVMD24 to 1 at locus VVMD28 and was higher than the expected one at 4 out of 11 loci. Microsatellite analysis proved to be useful for the genetic characterization of Romanian grapevines, allowing precise identification and good discrimination of all tested cultivars.

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Available abstract

The PCR-based DNA microsatellite analysis has been applied to define genetic relationships among eleven Romanian grapevine varieties. 87 alleles with a mean number of 7.90 alleles per locus were detected in the set of cultivars analyzed. The total number of alleles per locus varied between 4 (in VVMD24 and VVIQ52) and 10 (in VMC8G9, UDV125 and VMC5G6.1). The expected heterozygosity (gene diversity) ranged from 0.63 at locus VVMD24 to 0.92 at locus VVMD28. The observed heterozygosity varied between 0.63 at locus VVMD24 to 1 at locus VVMD28 and was higher than the expected one at 4 out of 11 loci. Microsatellite analysis proved to be useful for the genetic characterization of Romanian grapevines, allowing precise identification and good discrimination of all tested cultivars.

Key concepts: Locus (genetics), Microsatellite, Loss of heterozygosity, Biology, Allele, Genetic diversity, Genetics, Cultivar

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Genetic diversity of some Romanian grapevine cultivars as revealed by microsatellite markers. — Research Paper | ScholarLens