Antigenotoxic Effects of Brassica oleracea L. Var. Italica Aqueous Seed Extract on Allium cepa Root Chromosomal Aberration Assay

The herbicide cilluron containing cyhalofop-butyl (10%) is an active ingredient. Cyhalofop-butyl has low oral, dermal and inhalation toxicity. In the present study the antigenotoxic effects of Brassica oleracea L. var. italica was evaluated using Allium cepa root chromosomal aberration assay against ciluron. Three types of treatments were given to roots in pre-treatment roots were first treated with different concentrations (0.5, 1, 2, 4, 8 and 16 mg/ml) of extract for 3 hours followed by ciluron (0.8%). In post-treatment roots were first treated with ciluron followed by different concentrations of extract for 3 hours. In simultaneous treatment roots were treated with different concentrations of extract and ciluron simultaneously for 3 hours. The treatment of roots with distilled water and 0.8% ciluron served as negative and positive control. All the three types of treatments of extract showed dose dependent decrease in number of chromosomal aberrations. These results showed that Brassica oleracea L. var. italica aqueous seed extract have antigenotoxic effects.