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2010•Pakistan Journal of ScienceRequires access

PRETREATMENT OF SUGARCANE BAGASSE FOR THE BIOSYNTHESIS OF CELLULASES BY TRICHODERMA VIRIDE

Shuank Malik, Hamid Mukhtar, Ikram ul Haq

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Abstract

Present study describes the pretreatment of sugarcane bagasse with different physical and chemical agents for the production of cellulolyic enzymes by Trichoderma viride using submerged fermentation. Bagasse was pretreated with steam (121oC and 200oC) and different chemicals like NaOH, H2SO4, H2O2 and NaHClO3 to get the maximum yield of cellulases. Among all treatments, steam pretreatment at 200oC gave the maximum cellulases yield (CMCase 1.57 U/ml/min, FPase 0.921 U/ml/min) as compared to control (CMCase 0.084 U/ml/min, FPase 0.0138 U/ml/min), untreated bagasse. There was a considerable enhancement in the enzyme production in terms of several folds higher than control. The amount of sugarcane bagasse in the fermentation medium was also optimized and 1% level of bagasse was found to be optimum.

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What this paper is about

Present study describes the pretreatment of sugarcane bagasse with different physical and chemical agents for the production of cellulolyic enzymes by Trichoderma viride using submerged fermentation. Bagasse was pretreated with steam (121oC and 200oC) and different chemicals like NaOH, H2SO4, H2O2 and NaHClO3 to get the maximum yield of cellulases. Among all treatments, steam pretreatment at 200oC gave the maximum cellulases yield (CMCase 1.57 U/ml/min, FPase 0.921 U/ml/min) as compared to control (CMCase 0.084 U/ml/min, FPase 0.0138 U/ml/min), untreated bagasse. There was a considerable enhancement in the enzyme production in terms of several folds higher than control. The amount of sugarcane bagasse in the fermentation medium was also optimized and 1% level of bagasse was found to be optimum.

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Available abstract

Present study describes the pretreatment of sugarcane bagasse with different physical and chemical agents for the production of cellulolyic enzymes by Trichoderma viride using submerged fermentation. Bagasse was pretreated with steam (121oC and 200oC) and different chemicals like NaOH, H2SO4, H2O2 and NaHClO3 to get the maximum yield of cellulases. Among all treatments, steam pretreatment at 200oC gave the maximum cellulases yield (CMCase 1.57 U/ml/min, FPase 0.921 U/ml/min) as compared to control (CMCase 0.084 U/ml/min, FPase 0.0138 U/ml/min), untreated bagasse. There was a considerable enhancement in the enzyme production in terms of several folds higher than control. The amount of sugarcane bagasse in the fermentation medium was also optimized and 1% level of bagasse was found to be optimum.

Key concepts: Bagasse, Trichoderma viride, Cellulase, Chemistry, Fermentation, Yield (engineering), Food science, Botany

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PRETREATMENT OF SUGARCANE BAGASSE FOR THE BIOSYNTHESIS OF CELLULASES BY TRICHODERMA VIRIDE — Research Paper | ScholarLens