COST EFFECTIVE ISOCRATIC RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF ETORICOXIB AND PARACETAMOL IN PURE AND IN TABLET FORMULATION

The present paper describes the development and validation of a cost effective isocratic RP-HPLC method for simultaneous determination of etoricoxib and Paracetamol in pure and in tablet formulation as per ICH Norms. The chromatographic separations of the two drugs were analyzed on a Hypersil BDS C18 (250 x 4.6 mm i.d., 5μm particle size) column with mobile phase composed of 0.05 M sodium dihydrogen phosphate buffer (1% TEA, edjusted pH 3.2 with o-phosphoric acid): acetonitrile (35:65 v/v) at an injection volume of 20μl, flow rate of 1.0mL min -1 and UV detection was carried out at 235nm. Separation of both analytes was completed within 3 min. Calibration curves were linear with coefficient correlation between 0.9999 over a concentration range of 1000 to 3000µg/mL of etoricoxib and 1200 to 3600µg/mL for Paracetamol. The proposed RP-HPLC method was validated by determining its accuracy, precision and system suitability.