Simultaneous Determination of Rutin and Quercetin in Different parts of Capparis spinosa

Capparis spinosa is a multipurpose plant which contains a number of chemically active and diverse secondary metabolites, in particular, flavonoids. Rutin and quercetin are two major flavonoids in caper plant. In this study the HPLC method was developed for simultaneous determination of rutin and quercetin content in different parts of C.spinosa at the floral budding stage .Collection of plant materials were made from Tafresh, Iran. Plant were separated into root, stem, leaves, floral bud which were dried separately, and subsequently assayed for total rutin and quercetin content. The highest amount of rutin (25.82 mg/g) and quercetin (10.4 mg/g) was measured in the leaf of caper plant. The significant amounts of these antioxidants confirm the nutritional and medicinal value of caper.