Ciprofloxacin Resistance in Staphylococcus aureus and Pseudomonas aeruginosa Isolated from Patients in Baghdad
Mohammed F. Al Marjani, Kadhim A. Kadhim, Afraa Ali Kadhim, Sumayah Abdul-Hussein Ibraheem
Abstract
Mohammed F. Al Marjani, Kadhim A. Kadhim, Afraa Ali Kadhim, Sumayah Abdul-Hussein Ibraheem
Abstract
Total of 50 isolates of Pseudomonas aeruginosa and 50 isolates of Staphylococcus aureus were collected to detect the gyrA and qnrs among S aureus and Ps. aeruginosa isolates respectively from patients in Baghdad. The susceptibility to different antibiotics was evaluated by disk diffusion method and MICs of ciprofloxacin were determined. Among 50 Pseudomonas aeruginosa isolates, resistance was observed most often to Nalidixic acid (54%), followed by Levofloxacin (38%) and Norfloxacin (36%). The resistance patterns of S. aureus were Levofloxacin ( 20 %) , Norfloxacin ( 16 %) , Ofloxacin ( 18 %) ,Ciprofloxacin ( 16 %), Lomofloxacin ( 14 %) and Nalidixic acid ( 50 %). Pseudomonas aeruginosa isolates had MICs between (2-512) μg/ml and S. aureus isolates had MICs between (4-265) μg/ml. All S. aureus and Pseudomonas aeruginosa isolates were tested for the presence of the gyrA and qnrS genes by PCR, 36% of S. aureus and 24% of Pseudomonas aeruginosa isolates were positive respectively.
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Total of 50 isolates of Pseudomonas aeruginosa and 50 isolates of Staphylococcus aureus were collected to detect the gyrA and qnrs among S aureus and Ps. aeruginosa isolates respectively from patients in Baghdad. The susceptibility to different antibiotics was evaluated by disk diffusion method and MICs of ciprofloxacin were determined. Among 50 Pseudomonas aeruginosa isolates, resistance was observed most often to Nalidixic acid (54%), followed by Levofloxacin (38%) and Norfloxacin (36%). The resistance patterns of S. aureus were Levofloxacin ( 20 %) , Norfloxacin ( 16 %) , Ofloxacin ( 18 %) ,Ciprofloxacin ( 16 %), Lomofloxacin ( 14 %) and Nalidixic acid ( 50 %). Pseudomonas aeruginosa isolates had MICs between (2-512) μg/ml and S. aureus isolates had MICs between (4-265) μg/ml. All S. aureus and Pseudomonas aeruginosa isolates were tested for the presence of the gyrA and qnrS genes by PCR, 36% of S. aureus and 24% of Pseudomonas aeruginosa isolates were positive respectively.
Key concepts: Pseudomonas aeruginosa, Microbiology, Nalidixic acid, Ciprofloxacin, Staphylococcus aureus, Norfloxacin, Ofloxacin, Levofloxacin