1989•Unpublished venueRequires access
PURIFICATION AND SOME PROPERTIES OF A NOVEL ,8 - MANNOSIDASE PRODUCED BY A BACTERIUM ISOLATED FROM SOIL
Kaoru Takegawa, Fuminobu Osaka, Takayuki Jikibara, Shojiro Iwahara
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Abstract
A gram positive bacterium isolated from soil was found to produce 8 -mannosidase that act on 0-,4 D-mannopyranosyl-(1-2)-D-mannose in a cell-free extract The enzyme was partially purified from the cell-free extract to remove other glycosidase activities The opimum pH and temperature for the activity to ,4 -mannosidase was pH 6 5, and 40'C , respectively The enzyme was completely inhibited by Cu2+ and Hg2+, and strongly inhibited by the addition of mannose The enzyme almost completely hydrolyzed 0 8 D-mannopyranoslyl-(1-2)-D-mannose to mannose, but did not act on p-nitrophenyl8 -D-mannoside or 0 ,4 -