GENOMIC DNA ISOLATION FROM HUMAN WHOLE BLOOD SAMPLES BY NON ENZYMATIC SALTING OUT METHOD Original Article

Introduction: Human DNA can be extracted from all the nucleated cells such as hair, tissue, blood etc. certain sources contain high levels of proteins & many types of secondary metabolites that effects DNA purification, highly purified DNA is essential for molecular studies. Here we followed salting out method to extract large quantities of human DNA from whole blood. Methods: Blood sample was used for extraction of DNA by salting out method. Lysis Buffer contains detergent and salts which create a hypertonic condition resulting in lysis of cells. RBC lysis: by using TKM1 buffer, WBC lysis: by using TKM2 buffer, extraction of DNA & storage: by using SDS, Nacl&Tris EDTA buffer. Results: 1% Agarose gels were used to check the DNA by Gel dock. DNA quantified by using Spectrophotometer, quantity of DNA obtained from 300µl blood is 6 to 10ug/300ul. Conclusion: Using the Non enzymatic (Salting out) method, good quality DNA samples from a human whole blood can be extract that is enough to perform Polymerase chain reaction to study gene polymorphisms in human population.