1994•Unpublished venueRequires access
Early Serodiagnosis ofAcuteHumanCytomegalovirus Infection byEnzyme-Linked Immunosorbent AssayUsing Recombinant Antigens
Bodo Plachter, Walter Hinderer, Jacoba van Zanten, Gerhard Jahn, Abteilung Hamatologie
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Abstract
DNA fragments fromeight different reading frames ofhumancytomegalovirus (HCMV)were generated by PCRandsubsequently cloned andexpressed inEscherichia coli infusion withglutathione S-transferase. The recombinant viral antigens were evaluated inimmunoblot analyses. Themostreactive antigens werepurified andfurther evaluated inELISAs. Forthis, serafromhealthy blood donors andimmunocompetent individuals withacuteHCMV infection, andfollow-up serafromtransplant recipients withacuteprimary HCMV infection were used. Theresults ofour experiments indicate thatonlythree particular recombinant polypeptides from twoviral proteins arenecessaryforserodiagnosis. While a fragment coveringaminoacids(aa)495to691of ppl50(150/1) was themostsuitable antigen fortheidentification ofinfected individuals ingeneral, immunoglobulin M antibodies against theC-terminal partsofppl50(aa862to1048; 150/7) andp52(aa297 to433;52/3) proved tobeexcellent serological markers tomonitor acuteHCMV infection. Theselected recombinant antigens enable theimprovement ofserodiagnosis ofHCMV-related diseases, especially during theearly stages ofinfection.