Antioxidant activity of Salvia jurisicii Košanin ethanol extracts.
Ana Alimpić Aradski, Sonja Duletić‐Laušević, Vlado Matevski, Petar D. Marin
Abstract
Ana Alimpić Aradski, Sonja Duletić‐Laušević, Vlado Matevski, Petar D. Marin
Abstract
In this study, ethanol extracts (96%, 50%, 30% and 10%) of Salvia jurisicii Kosanin whole plant and different parts (leaves and stems) were investigated for in vitro antioxidant 2,2-diphenyl-1- picrylhydrazyl (DPPH) and 2,2ʹ-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid diammonium salt (ABTS) free radical-scavenging activity and total phenolic and flavonoid contents. The largest extract yields were obtained from the 50% and 30% leaf extracts (21.22% and 19.85%, respectively). The 50% and 96% ethanol extracts of whole plants showed the highest activities against DPPH radical (97.1 and 100.6 µg/ml, respectively) whereas leaf extracts were superior in the ABTS test (1.76 and 1.65 mg AAE/g, for 96% and 50%, respectively). Total phenolic and flavonoid contents were ranged in similar orders for the various extracts: 96%>50%>30%>10%. Ethanol extracts of leaves were the richest in phenols and flavonoids, followed by the whole plant and stems. A very strong linear correlation between ABTS activity and phenolic content of extracts was established (r=0.932). The results suggest that the herb Salvia jurisicii , particularly the leaves, could be considered as a potential source of natural antioxidants.
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In this study, ethanol extracts (96%, 50%, 30% and 10%) of Salvia jurisicii Kosanin whole plant and different parts (leaves and stems) were investigated for in vitro antioxidant 2,2-diphenyl-1- picrylhydrazyl (DPPH) and 2,2ʹ-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid diammonium salt (ABTS) free radical-scavenging activity and total phenolic and flavonoid contents. The largest extract yields were obtained from the 50% and 30% leaf extracts (21.22% and 19.85%, respectively). The 50% and 96% ethanol extracts of whole plants showed the highest activities against DPPH radical (97.1 and 100.6 µg/ml, respectively) whereas leaf extracts were superior in the ABTS test (1.76 and 1.65 mg AAE/g, for 96% and 50%, respectively). Total phenolic and flavonoid contents were ranged in similar orders for the various extracts: 96%>50%>30%>10%. Ethanol extracts of leaves were the richest in phenols and flavonoids, followed by the whole plant and stems. A very strong linear correlation between ABTS activity and phenolic content of extracts was established (r=0.932). The results suggest that the herb Salvia jurisicii , particularly the leaves, could be considered as a potential source of natural antioxidants.
Key concepts: ABTS, DPPH, Flavonoid, Chemistry, Antioxidant, Phenols, Ethanol, Food science