2010•Acta Horticulturae SinicaRequires access
Studies on transcriptional level of VvNCED1 and VvBG1, two key genes related to ABA accumulation around the veraison of grape berry ripening.
Yanshu Wang, Chunli Li, Haifeng Jia, Ling Qin, Gao DongSheng, Shen YuanYue
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Abstract
With Hamburg grape mesocarp from berries during 7–11 weeks after flowering,the expression levels of VvNCED1 and VvBG1 gene were detected through real-time quantitative PCR.The results showed that the expression level of VvNCED1 gene was very high during 7–8 weeks after flowering,followed by a rapid decline to its lowest point,and then increased a little.Coupled with the onset of berry ripening and coloring-up,the mRNA expression of VvBG1 gene,on the whole,increased continually.Given that the ABA continual rising during the onset of grape berry ripening,it is considered that,the ABA accumulation is attributed to high mRNA expression level of VvNCED1 before the onset of berry ripening and high mRNA expression level of VvBG1 after the onset of berry ripening.