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Concepts in Imaging and Microscopy Choosing Objective Lenses: The Importance of Numerical Aperture and Magnification in Digital Optical Microscopy

David W. Piston

Abstract

Microscopic images are characterized by a number of microscope-specific parametersâ€numerical ap erture (NA), magnification (M), and resolution (R)â€and by parameters that also depend on the specimenâ€for ex ample, contrast, signal-to-noise ratio, dynamic range, and integration time. In this article, issues associated with the microscope-specific parametersNA, M, and R are discussed

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Key concepts: Magnification, Numerical aperture, Microscopy, Microscope, Optics, Optical microscope, Resolution (logic), Digital holographic microscopy