Measurement of zinc, iron and manganese ratios in different parts of soybean plant

The effects of zinc, iron and manganese on their ratio in stem, leaf, pod and seed at reproductive growth stages of soybean (Glycine max L.) were studied in field and pot experiments. Three rates of zinc (0, 4 and 8 mg Zn/kg as ZnSO 4 .7H 2 O), iron (0, 4 and 8 mg Fe/kg as FeSO 4 ) and manganese (0, 15 and 30 mg Mn/kg as MnSO 4 .4H 2 O) in pot experiment, and zinc (0, 20 and 40 kg/ha from ZnSO 4 source), iron (0, 25 and 50 kg/ha from FeSO 4 source) and manganese (0, 25 and 40 kg/ha from MnSO 4 source) in field conditions were used. The results showed that zinc, iron and manganese were transferred from the stem to leaves when that increasing soybean old and approach to maturity stage (R 8 ). With application of Zn, Fe and Mn fertilizers, [Zn] leaf /[Zn] stem ratio increased from the R 1 to R 8 . In contrast, [Zn] pod /[Zn] stem ratio was decreased from R 6 to R 8 growth stages. Manganese fertilization causing that Fe transferring was increased from the stem, leaf and pod to seeds. Iron ratio in leaf and stem decreased from R 1 to R 3 growth stages, and then increased thereto for R 6 stage. A similar trend was observed in case of [Zn] pod /[Zn] stem and [Mn] pod /[Mn] stem ratios from the R 6 to R 8 growth stages. With manganese fertilization [Mn] seed /[Mn] stem , [Mn] seed /[Mn] leaf and [Mn] seed /[Mn] pod increased sharply. The largest of degrees translocation factor belonged to manganese than the zinc and iron.