2014•Unpublished venueRequires access

Isolation, Purification and Characterization of Glucanase Enzyme from the Antagonistic Fungus Trichoderma

Sonika Pandey, Mohammad Hasan Shahid, Mukesh Srivastava, Antima Sharma, Anuradha Singh, Vipul Kumar

Abstract

Glucanase enzyme isolated from Trichoderma isolates were purified to homogeneity using ammonium persulphate precipitation and Fast Protein Liquid Chromatography. Purity of the isolated enzyme was confirmed by SDS-PAGE. Enzymatic properties such as effect of temperature, pH, SDS and EDTA were determined. Molecular weight of Trichoderma isolates was found to be around 55 kDa. The optimum temperature for glucanase enzyme was 50˚C and the optimum pH was 5.0. SDS and EDTA were at a concentration 0f 20 µg/ml showing an inhibitory effect on glucanase enzyme activity.

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Key concepts: Glucanase, Enzyme, Trichoderma, Chemistry, Enzyme assay, Chromatography, Fungus, Ammonium sulfate precipitation