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1997•Journal of General VirologyRequires access

Tubule-forming capacity of the movement proteins of alfalfa mosaic virus and brome mosaic virus.

D. Kasteel, Rob Goldbach, K.A.J. Jansen, J W van Lent, Nicole N. van der Wel

Open publisher page 104 citations

Abstract

The structural phenotype of the movement proteins (MPs) of two representatives of the Bromoviridae, alfalfa mosaic virus (AMV) and brome mosaic virus (BMV), was studied in protoplasts. Immunofluorescence microscopy showed that the MPs of these viruses, for which there has been no evidence of a tubule-guided mechanism, assemble into long tubular structures at the surface of the infected protoplast. Electron microscopy and immunogold analysis confirmed the presence of both MP and virus particles in the tubules induced by AMV and BMV. The significance of the tubule-forming properties of these viral MPs is discussed.

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What this paper is about

The structural phenotype of the movement proteins (MPs) of two representatives of the Bromoviridae, alfalfa mosaic virus (AMV) and brome mosaic virus (BMV), was studied in protoplasts. Immunofluorescence microscopy showed that the MPs of these viruses, for which there has been no evidence of a tubule-guided mechanism, assemble into long tubular structures at the surface of the infected protoplast. Electron microscopy and immunogold analysis confirmed the presence of both MP and virus particles in the tubules induced by AMV and BMV. The significance of the tubule-forming properties of these viral MPs is discussed.

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OpenAlex reports 104 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

The structural phenotype of the movement proteins (MPs) of two representatives of the Bromoviridae, alfalfa mosaic virus (AMV) and brome mosaic virus (BMV), was studied in protoplasts. Immunofluorescence microscopy showed that the MPs of these viruses, for which there has been no evidence of a tubule-guided mechanism, assemble into long tubular structures at the surface of the infected protoplast. Electron microscopy and immunogold analysis confirmed the presence of both MP and virus particles in the tubules induced by AMV and BMV. The significance of the tubule-forming properties of these viral MPs is discussed.

Key concepts: Brome mosaic virus, Immunogold labelling, Biology, Alfalfa mosaic virus, Virus, Virology, Immunofluorescence, Tubule

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Tubule-forming capacity of the movement proteins of alfalfa mosaic virus and brome mosaic virus. — Research Paper | ScholarLens