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CORRELATION BETWEEN PATHOLOGICAL FINDINGS AND BACTERIOLOGICAL CULTURE ON PARATUBERCULOUS CATTLE

Paula Ristow, Ana Bárbara, Freitas Rodrigues, Leila Souza Fonseca, Walter Martin Roland, Guilherme Nunes de Souza, Carla Dray Marassi, Queiroz de Carvalho, Walter Lilenbaum

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Abstract

ABSTRACT - Paratuberculosis (PTB) is a chronic enteritis determined by Mycobacterium avium subsp. paratuberculosis (Map) that affects ruminants. Three crossbred dairy cattle with clinical PTB were necropsied; tissue samples were collected and processed for histopathology and culture. Intestinal lesions were characterized in three levels of severity (mild, moderate and severe) by gross examination. Gross examination, microscopy and tissue culture were analyzed and compared. Mean tissue culture sensitivity was 66.6%. Map was recovered from 42.8% of intestinal mild lesions, 55.5% of intestinal moderate lesions, and 100% of severe intestine lesions. The results indicate a significant correlation between the level of lesions severity and the isolation of Map.

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ABSTRACT - Paratuberculosis (PTB) is a chronic enteritis determined by Mycobacterium avium subsp. paratuberculosis (Map) that affects ruminants. Three crossbred dairy cattle with clinical PTB were necropsied; tissue samples were collected and processed for histopathology and culture. Intestinal lesions were characterized in three levels of severity (mild, moderate and severe) by gross examination. Gross examination, microscopy and tissue culture were analyzed and compared. Mean tissue culture sensitivity was 66.6%. Map was recovered from 42.8% of intestinal mild lesions, 55.5% of intestinal moderate lesions, and 100% of severe intestine lesions. The results indicate a significant correlation between the level of lesions severity and the isolation of Map.

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Available abstract

ABSTRACT - Paratuberculosis (PTB) is a chronic enteritis determined by Mycobacterium avium subsp. paratuberculosis (Map) that affects ruminants. Three crossbred dairy cattle with clinical PTB were necropsied; tissue samples were collected and processed for histopathology and culture. Intestinal lesions were characterized in three levels of severity (mild, moderate and severe) by gross examination. Gross examination, microscopy and tissue culture were analyzed and compared. Mean tissue culture sensitivity was 66.6%. Map was recovered from 42.8% of intestinal mild lesions, 55.5% of intestinal moderate lesions, and 100% of severe intestine lesions. The results indicate a significant correlation between the level of lesions severity and the isolation of Map.

Key concepts: Paratuberculosis, Histopathology, Enteritis, Gross examination, Pathology, Pathological, Biology, Mycobacterium

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