SCREENING OF ANTIFUNGAL ACTIVITY OF PLEUROTUS OSTREATUS AND AGARICUS BISPORUS

ABSTRACT The fruiting body powder of Pleurotus ostreatus (oyster mushroom) and Agaricus bisporus (button mushroom) was extracted with ethanol by using the agar well diffusion technique, the extract were tested for their antifungal activity against six fungi belonging to 5 genera viz. Aspergillus flavus, Aspergillus fumigatous, Penicillium chrysogenum, Sporotricum carnis, Humicola grisea and Thermoascus aurantiacus. The extract of Pleurotus ostreatus showed the maximum inhibition against Penicillium chrysogenum and minimum against Aspergillus flavus. The extract of Agaricus bisporus showed the maximum inhibition against Humicola grisea and minimum against same fungi Aspergillus flavu Key Words:- Antifungal activity, Fruiting Body, Pleurotus ostreatus , Agaricus Bisporus, ethanol extract REFERENCES Amuneke EH, Dike KS, and Ogbulie JN, 2011. Cultivation of Pleurotus ostreatus: An edible mushroom from agro base waste products. J. Microbiol. Biotech. Res., Vol. 1 (3):1-14. Aneja KR, 2001. Experiments in Microbiology. Plant pathology and Biotechnology, Newage International Publishers. Vol 4:157-162. Barros L, Calhella RC and Vaz JA, 2007. Antimicrobial activity and bioactive compounds of Portugese wild edible mushrooms methanolic extracts. European Food Research and Technology. 225: 151-156. Bender S, Lonergan GT, Backhaus J, Dumitrache A andBaker WL, 2001. The antibacterial activity of the edible and medicinal mushroom Lentinus edodes (Berk.) Sing. Int. J. Med. Mushrooms 3: 118. Benedict RG and Brady LR, 1972. Antimicrobial activity of mushroom metabolites. J. Pharm. Sci. 61: 1820-21. Chang ST and Miles Philip G, 2004. Mushrooms Cultivation, Nutritional Value, Medicinal Effect, and Environmental Impact. 2nd Edn.CRC Press.USA. Cohen R, Persky L and Hadar Y, 2002. Biotechnological applications and potential of wood-degrading mushrooms of the genus Pleurotus. Appl. Microbiol. Biotechnol. 58: 582-594. Demirhan A, Yesil OF, Yildiz A and Gul K, 2007. A research on antimicrobial activity of some macrofungi species. Science and Engineering Journal of Firat University. 19: 425-433. Gbolagade JS and Fasidi IO, 2005. Antimicrobial activities of some selectedNigerian mushrooms. 8: 83-87. Gilman JC, 2001. A Manual of Soil fungi. Biotech Books, 2nd Indian edition, Delhi. Jonathan SG and Fasidi JO, 2003. Antimicrobial activities of two Nigerian edible macro-fungi Lycoperdon pusilum (Bat. Ex) and Lycoperdon giganteum (Pers). African.J.Biomed.Res. 6: 85-90. Nagamani A, Kunwar IK and Manoharachary C, 2006. Hand book of soil fungi. I.K. International Pvt. Ltd. Nehra Kiran, Meenakshi, Kumar Mukesh and Yadav Ajay, 2012. Evaluation of antimicrobial potential of fruiting body extracts of Pleurotus ostreatus (oyster mushroom). International Journal of Microbial Resource Technology.Vol.1,4-2278 – 3822. Ngai PHK and Ng TB, 2004. A ribonuclease with antimicrobial, antimitogenic and antiproliferative activities from the edible mushroom Pleurotus sajor-caju. Peptides 25: 11-17. Richa, Shikha Sharma and M.L. Sharma Phytochemical Investigations And Anatomical Study Of Three Species Of Sida. Biolife. 2(2):622-629. Solak MH, Kalmis E, Saglam H and Kalyoncu F, 2006. Antimicrobial activity of two wild mushrooms Clitocybe alexandri (Gill.) Konr. and Rhizopogon roseolus (Corda) T.M. Fries collected from Turkey. Phytotherapy Research. 20: 1085-1087. Tochikura TS, Nakashima H, Ohashi Y and Yamamoto N, 1988. Inhibition (in- vitro) of replication and of the cytopathic effect of human immunodeficiency virus by an extract of the culture medium of Lentinus edodes mycelia. Med. Microbiol. Imm. 177: 235-244. Turkoglu A, Kivrak I, Mercan N, Duru ME, Gezer K and H. Turkoglu H, 2006. Antioxidant and antimicrobial activities of Morchella conica. Pers. African Journal of Biotechnol. 5: 1146-1150. Waksman SA, 1922. A method of counting the number of fungi in the soil. J. Bact. 7: 339-341. Wang HX and Ng TB, 2000. Isolation of a novel ubiquitin-like protein from Pleurotus ostreatus mushroom with anti-humanimmunodeficiency virus, translation-inhibitory and ribonuclease activities. . 276: 587-93. Wang HX, Ng TB and Liu QH, 2004. Alveolarin, a novel antifungal polypeptide from the wild mushroom Polyporus alveolaris. Peptides 25: 693–696. Wang J, Wang HX and Ng TB, 2007. A peptide with HIV-1 reverse transcriptase inhibitory activity from the medicinal mushroom Russula paludosa. Peptides 28: 560-65.