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2013•International Journal of Animal and Veterinary AdvancesOpen access

Development of Duplex PCR Assay for the Detection of Mycoplasma Gallisepticum and Mycoplasma Synoviae Prevalence in Pakistan

Abida Arshad, Khaleeq-Uz- Zaman, Abida Arshad, Khaleeq-Uz-Zaman, Ijaz Ali, Muhammad Saad Ahmed, Mukhtar Alam, Ahmad ur Rehman Saljoqi, Aqeel Javed, Naeemud Din Ahmadand, Zahoor Ahmad Swati

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Abstract

Mycoplasma infections are of great concern in avian medicine, because they cause economic losses in commercial poultry production of Pakistan. Timely and efficient diagnosis of the mycoplasma is needed in order to practice prevention and control strategies. A duplex Polymerase Chain Reaction (PCR) was optimized for simultaneous detection of two pathogenic species of avian mycoplasmas. Two sets of oligonucleotide primers specific for Mycoplasma gallisepticum and Mycoplasma synoviae, were used in the test. The developed assay exhibited high sensitivity and 100% specificity for the simultaneous detection of mycoplasma species prevalence in Pakistan.

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What this paper is about

Mycoplasma infections are of great concern in avian medicine, because they cause economic losses in commercial poultry production of Pakistan. Timely and efficient diagnosis of the mycoplasma is needed in order to practice prevention and control strategies. A duplex Polymerase Chain Reaction (PCR) was optimized for simultaneous detection of two pathogenic species of avian mycoplasmas. Two sets of oligonucleotide primers specific for Mycoplasma gallisepticum and Mycoplasma synoviae, were used in the test. The developed assay exhibited high sensitivity and 100% specificity for the simultaneous detection of mycoplasma species prevalence in Pakistan.

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Available abstract

Mycoplasma infections are of great concern in avian medicine, because they cause economic losses in commercial poultry production of Pakistan. Timely and efficient diagnosis of the mycoplasma is needed in order to practice prevention and control strategies. A duplex Polymerase Chain Reaction (PCR) was optimized for simultaneous detection of two pathogenic species of avian mycoplasmas. Two sets of oligonucleotide primers specific for Mycoplasma gallisepticum and Mycoplasma synoviae, were used in the test. The developed assay exhibited high sensitivity and 100% specificity for the simultaneous detection of mycoplasma species prevalence in Pakistan.

Key concepts: Mycoplasma synoviae, Mycoplasma gallisepticum, Mycoplasma, Biology, Polymerase chain reaction, Microbiology, Virology, Gene

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Development of Duplex PCR Assay for the Detection of Mycoplasma Gallisepticum and Mycoplasma Synoviae Prevalence in Pakistan — Research Paper | ScholarLens