The Antigenicity of Avian Influenza a H5n1 Protein with Anti H5n1 and H5n2 Antibody Avian Influenza

2 Abstract: Avian influenza virus H5N1 causes a zoonotic disease, that is, Avian influenza, that can potentially infect humans, wild birds and other mammals. A rapid diagnosis and vaccination against this virus is important to control the disease outbreak. However, information about the whole protein profile as the basic information for developing vaccine and making diagnosis of Avian influenza H5N1 virus is still limited. Furthermore, we need to observe the acurate protein profile of the virus. The purpose of this research was to identify protein profile of H5N1 and its antigenicity to H5N1 and H5N2 chicken sera antibody. We employed SDS PAGE to identify the protein profile of Avian influenza A H5N1 isolated from infected chicken in the field. Western Blott was used to identify antigenic activity of the H5N1 protein against antibody of vaccinated chicken by H5N1 and H5N2. The result showed that the protein profile of Avian influenza A H5N1 consisted of PB1, PB2, PA, HA, NP, NA and M1 with molecular weight of 86.5, 85.8, 82.5, 63.5, 56.2, 49.7 and 27.9 kDa, respectively. Here we identified that protein HA and NA were recognized by H5N1 and H5N2 sera antibody that are very promising for the development of vaccines and diagnostic materials of Avian influenza A Virus.