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IN VITRO ANTIOXIDANT AND ANTI-ARTHRITIC ACTIVITIES OF SHILAJIT

Anuya Aparna Rege, Parikshit Juvekar, Archana R. Juvekar

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Abstract

The aim of present in vitro study was to investigate antioxidant and anti-arthritic activities of Shilajit . The antioxidant activity of aqueous extract of Shilajit was determined by using 3 in vitro parameters, namely, DPPH radical-scavenging assay, lipid peroxidation inhibitory assay and reducing power assay, whereas, anti-arthritic activity was evaluated by proteinase inhibitory assay. Besides, phenolic content of the extract was estimated using Folin-Ciocalteu method. Aqueous extract of Shilajit exhibited DPPH radical-scavenging activity with IC50 value of 11.9µg/ml, which was similar to that of standard ascorbic acid. Its reductive ability increased with increasing concentration which was comparable to that of standard. It also showed significant (P<0.05) lipid peroxidation inhibitory and anti-arthritic activities. High phenolic content of Shilajit can be correlated to its respective antioxidant and anti-arthritic activities.

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What this paper is about

The aim of present in vitro study was to investigate antioxidant and anti-arthritic activities of Shilajit . The antioxidant activity of aqueous extract of Shilajit was determined by using 3 in vitro parameters, namely, DPPH radical-scavenging assay, lipid peroxidation inhibitory assay and reducing power assay, whereas, anti-arthritic activity was evaluated by proteinase inhibitory assay. Besides, phenolic content of the extract was estimated using Folin-Ciocalteu method. Aqueous extract of Shilajit exhibited DPPH radical-scavenging activity with IC50 value of 11.9µg/ml, which was similar to that of standard ascorbic acid. Its reductive ability increased with increasing concentration which was comparable to that of standard. It also showed significant (P<0.05) lipid peroxidation inhibitory and anti-arthritic activities. High phenolic content of Shilajit can be correlated to its respective antioxidant and anti-arthritic activities.

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Available abstract

The aim of present in vitro study was to investigate antioxidant and anti-arthritic activities of Shilajit . The antioxidant activity of aqueous extract of Shilajit was determined by using 3 in vitro parameters, namely, DPPH radical-scavenging assay, lipid peroxidation inhibitory assay and reducing power assay, whereas, anti-arthritic activity was evaluated by proteinase inhibitory assay. Besides, phenolic content of the extract was estimated using Folin-Ciocalteu method. Aqueous extract of Shilajit exhibited DPPH radical-scavenging activity with IC50 value of 11.9µg/ml, which was similar to that of standard ascorbic acid. Its reductive ability increased with increasing concentration which was comparable to that of standard. It also showed significant (P<0.05) lipid peroxidation inhibitory and anti-arthritic activities. High phenolic content of Shilajit can be correlated to its respective antioxidant and anti-arthritic activities.

Key concepts: Antioxidant, Chemistry, DPPH, Ascorbic acid, Lipid peroxidation, IC50, In vitro, Biochemistry

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