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PRODUCTION OF COLLAGENASE INHIBITOR BY MOUSE CALVARIA IN TISSUE CULTURE

Masaru Nagayama, Seizaburo Sakamoto

Abstract

A collagenase inhibitor was identified in the culture medium of mouse calvaria after separation from mouse bone collagenase by Sephadex G-200 gel filtration equilibrated with 6M urea in Tris buffer. The simultaneous synthesis of both collagenase and inhibitor by mouse calvaria in tissue culture was consistent with the resuls of our previous study using a chick bone culture system and otehr recent studies.

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Available abstract

Key concepts: Calvaria, Collagenase, Sephadex, Chemistry, Tissue culture, Molecular biology, Biochemistry, Biology