Preliminary Phytochemical Screening and Evaluation of In vitro Antioxidant Activity of Anthocephalous cadamba by Using Solvent Extracts
V. Alekhya, Thiyagarajan Deepan
Abstract
V. Alekhya, Thiyagarajan Deepan
Abstract
The present study was to estimate preliminary photochemical evaluation and in vitro antioxidant of leaves and fruit extracts of anthocephalous cadamba by using different solvents like pet ether, ethanol, chloroform and water. Preliminary phytochemical analysis reveals the presence of carbohydrates, tannins, saponins, flavanoids and terpenoids. The extract was screened for its potential antioxidant activity using DPPH free radical scavenging activity. The reducing power extract was also determined ascorbic acid was used as a standard and positive control for both leaves and fruit analysis. All the analysis was made by using UV spectrophotometer (Perkin Elmer). The ethanol leaves and fruit extract shown significant shown DPPH (1, 1-Diphenyl-2-picryl-hydrazyl) radical scavenging activity compared to standard antioxidant. The DPPH radical scavenging activity of the extract was increased with increasing concentration. In DPPH free radical scavenging assay IC values of leaf and fruit extract of anthocephalous cadamba was found to be 22 and 18 50 µg/ml respectively. The results were concluded that extracts have a potential source of antioxidants of natural origin.
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The present study was to estimate preliminary photochemical evaluation and in vitro antioxidant of leaves and fruit extracts of anthocephalous cadamba by using different solvents like pet ether, ethanol, chloroform and water. Preliminary phytochemical analysis reveals the presence of carbohydrates, tannins, saponins, flavanoids and terpenoids. The extract was screened for its potential antioxidant activity using DPPH free radical scavenging activity. The reducing power extract was also determined ascorbic acid was used as a standard and positive control for both leaves and fruit analysis. All the analysis was made by using UV spectrophotometer (Perkin Elmer). The ethanol leaves and fruit extract shown significant shown DPPH (1, 1-Diphenyl-2-picryl-hydrazyl) radical scavenging activity compared to standard antioxidant. The DPPH radical scavenging activity of the extract was increased with increasing concentration. In DPPH free radical scavenging assay IC values of leaf and fruit extract of anthocephalous cadamba was found to be 22 and 18 50 µg/ml respectively. The results were concluded that extracts have a potential source of antioxidants of natural origin.
Key concepts: DPPH, Chemistry, Phytochemical, Antioxidant, Ascorbic acid, Terpenoid, Ethanol, Chloroform