Analysis of genetic polymorphisms in vegetable crops of Brassica campestris by RAPD markers
Chen Yunpeng, Cao Jia-shu, Miao Ying, Wanzhi Ye
Abstract
Chen Yunpeng, Cao Jia-shu, Miao Ying, Wanzhi Ye
Abstract
The genetic polymorphisms in vegetable crops of Brassica campestris was investigated using random amplified polymorphic DNA markers (RAPDs), the detected materials included thirty-three cultivars. Thirty-seven primers selected from seventy primers amplified 353 RAPD fragments and 260 bands of them showed polymorphisms. The length of most amplified fragments ranged from 0.9 to 1.6 kb. Different primers exhibited different detective efficiency. There were ten RAPD characteristic markers, which were detected as a group of DNA fingerprints to identify all the seven cultivars of heading type Chinese cabbage.
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The genetic polymorphisms in vegetable crops of Brassica campestris was investigated using random amplified polymorphic DNA markers (RAPDs), the detected materials included thirty-three cultivars. Thirty-seven primers selected from seventy primers amplified 353 RAPD fragments and 260 bands of them showed polymorphisms. The length of most amplified fragments ranged from 0.9 to 1.6 kb. Different primers exhibited different detective efficiency. There were ten RAPD characteristic markers, which were detected as a group of DNA fingerprints to identify all the seven cultivars of heading type Chinese cabbage.
Key concepts: RAPD, Brassica, Biology, Genetic marker, Cultivar, Genetics, Botany, Gene