Effect of Particulate Matter 2.5 on the Proliferation and Secretion of NO,ET-1 in Vascular Smooth Muscle Cell of Rats in Vitro

Objective To observe the effect of PM2.5 on the secretion of NO, ET-1 and the proliferation of rat vascular smooth muscle cell(VSMC) in vitro.Methods VSMCs were treated with PM2.5 collected from urban area of Zhanjiang,at the doses of 10,20,50,100 and 200 μg/ml.After 24 hours of treatment, the levels of NO and ET-1 in the VSMCs were detected by nitrate reductase method and radioimmunoassay respectively, the proliferation of VSMCs was detected by MTT.Results At the concentrations of 10-200 μg/ml, with PM2.5 increased, the level of NO in VSMCs decreased from(43.53 ±3.46)μmol/L to(29.28±2.28)μmol/L(F=18.89,P0.01),meanwhile, the level of ET-1 increased from(38.06±3.53)ng/L to(112.93±15.63)ng/L(F=22.39,P0.01),MTT value increased from 0.49±0.067 to 0.72±0.025(F=27.89,P0.01).Conclusion In VSMC, PM2.5 can inhibite the secretion of NO, promote the secretion of ET-1 and activate cell proliferation in vitro.