Purification and Characterization of an Antimicrobial Substance from Bacillus subtilis HH28 Antagonistic to Bacillus cereus

泥�援��옣�쑝濡쒕��꽣 Bacillus cereus �뿉 ���븳 �빆洹좏솢�꽦�씠 媛��옣 �넂�� 洹좎＜瑜� 遺꾨━�븯�뿬 �삎�깭�븰�쟻, �깮�솕�븰�쟻 �듅�꽦怨� 16S rDNA �뿼湲곗꽌�뿴 寃곗젙�쓣 �넻�빐 Bacillus subtilis HH28�쑝濡� �룞�젙 諛� 紐낅챸�븯���떎. B. subilis HH28�쓽 �깮�쑁�떆湲곗뿉 �뵲瑜� �빆洹좏솢�꽦�쓣 痢≪젙�빐 蹂� 寃곌낵, �깮�쑁�씠 ���닔利앹떇湲곗씤 9�떆媛꾨��꽣 �깮�꽦�릺�뼱 �궗硫멸린�씤 60�떆媛꾩뿉 媛��옣 �넂�� �솢�꽦(80 AU/ml)�쓣 �굹���궡�뿀怨� 144�떆媛�(6�씪)源뚯� �빆洹좏솢�꽦�쓣 �쑀吏��븯���떎. �빆洹좊Ъ吏덉쓽 �젙�젣�뒗 �솴�궛�븫紐⑤뒆 移⑥쟾怨� DEAE-sepharose fast flow, sephacryl S-200HR瑜� �씠�슜�븯��怨�, 19.7諛곗쓽 �젙�젣�룄�� 38.4%�쓽 �닔�쑉濡� �젙�젣�릺�뿀�떎. Tricine SDS-PAGE�� directed detection�쓣 �넻�빐 �빆洹좊Ъ吏덉쓽 遺꾩옄�웾�씠 �빟 3,500 Da�엫�쓣 �븣 �닔 �엳�뿀�떎. �삉�븳 蹂� �뿰援ъ쓽 �빆洹좊Ъ吏덉쓣 B. cereus 肉먮쭔 �븘�땲�씪 Listeria monocytogenes, Vibrio parahaemolyticus �쓽 �떇以묐룆 洹좎뿉�꽌�룄 �슦�닔�븳 �빆洹좏솢�꽦�쓣 �굹���궡�뿀怨�, �빆洹좎옉�슜�쓽 湲곗옉�� 誘몄깮臾쇱쓣 �궗硫몄떆�궎�뒗 �궡洹좎옉�슜�씠���떎. �삉�븳 �삩�룄 �븞�젙�꽦 �떎�뿕�뿉�꽌 40-80�꼦源뚯� �븞�젙�뻽怨�, pH �븞�젙�꽦 �떎�뿕�뿉�꽌�뒗 pH 2-9源뚯� �븞�젙�븯�뿬 鍮꾧탳�쟻 �삩�룄�� pH�뿉 �븞�젙�븯���떎. �슚�냼�뿉 ���븳 �쁺�뼢 �떎�뿕�뿉�꽌�뒗 �떒諛깆쭏 遺꾪빐�슚�냼�뿉 �쓽�빐 �빆洹좏솢�꽦�씠 �떎�솢�릺�뼱 蹂� �뿰援ъ쓽 �빆洹좊Ъ吏덉� �떒諛깆쭏�꽦�엫�쓣 �븣 �닔 �엳�뿀�떎. �쐞�� 媛숈� �듅�꽦�쑝濡� 蹂댁븘 B. subtilis HH28�씠 �깮�궛�븯�뒗 �빆洹좊Ъ吏덉� 泥쒖뿰 �떇�뭹 蹂댁〈�젣 諛� �궗猷� 蹂댁〈�젣, �빆�깮�젣 ��泥� �쓽�빟�뭹�쑝濡� �궗�슜�븷 �닔 �엳�뒗 �옞�옱�젰�쓣 媛�吏�怨� �엳�쑝硫�, �뼢�썑 �씠 �빆洹좊Ъ吏덉쓽 �젙�솗�븳 援ъ“ 諛� �듅�꽦 洹쒕챸 �벑�쓽 �뿰援ш� �븘�슂�븯�떎