2009Journal of Plant Protection ResearchOpen access

Mycotoxins Biosynthesis by Fusarium Oxysporum and F. Proliferatum Isolates of Asparagus Origin

Agnieszka Waśkiewicz, Lidia Irzykowska, Z. Karolewski, Jan Bocianowski, Piotr Goliński, Z. Weber

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Abstract

The subject of this work was fumonisin B 1 (FB 1 ) and moniliformin (MON) biosynthesis by three isolates of Fusarium oxysporum and three isolates of F. proliferatum of asparagus spears origin.The cultures of fungi were grown on rice and asparagus media for 3 weeks at 20 o C. Experiment was carried out in 3 replicates.FB 1 and MON occurrence was evaluated with high-performance liquid chromatography (HPLC) analyses.Analysis of variance was carried out to determine biosynthesis of FB 1 and MON by F. oxysporum and F. proliferatum.FB 1 was found in the amount up to 2012.8 µg/g in cultures of F. proliferatum on rice and in a very small amount in two cultures on asparagus medium.F. oxysporum did not produce FB 1 on any of the media.MON was biosynthesized by two the same isolates of F. oxysporum in the amount up to 182.8 µg/g on rice and up to 743.3 µg/g on asparagus medium and one isolate (different on each medium) of F. proliferatum.

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The subject of this work was fumonisin B 1 (FB 1 ) and moniliformin (MON) biosynthesis by three isolates of Fusarium oxysporum and three isolates of F. proliferatum of asparagus spears origin.The cultures of fungi were grown on rice and asparagus media for 3 weeks at 20 o C. Experiment was carried out in 3 replicates.FB 1 and MON occurrence was evaluated with high-performance liquid chromatography (HPLC) analyses.Analysis of variance was carried out to determine biosynthesis of FB 1 and MON by F. oxysporum and F. proliferatum.FB 1 was found in the amount up to 2012.8 µg/g in cultures of F. proliferatum on rice and in a very small amount in two cultures on asparagus medium.F. oxysporum did not produce FB 1 on any of the media.MON was biosynthesized by two the same isolates of F. oxysporum in the amount up to 182.8 µg/g on rice and up to 743.3 µg/g on asparagus medium and one isolate (different on each medium) of F. proliferatum.

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Available abstract

The subject of this work was fumonisin B 1 (FB 1 ) and moniliformin (MON) biosynthesis by three isolates of Fusarium oxysporum and three isolates of F. proliferatum of asparagus spears origin.The cultures of fungi were grown on rice and asparagus media for 3 weeks at 20 o C. Experiment was carried out in 3 replicates.FB 1 and MON occurrence was evaluated with high-performance liquid chromatography (HPLC) analyses.Analysis of variance was carried out to determine biosynthesis of FB 1 and MON by F. oxysporum and F. proliferatum.FB 1 was found in the amount up to 2012.8 µg/g in cultures of F. proliferatum on rice and in a very small amount in two cultures on asparagus medium.F. oxysporum did not produce FB 1 on any of the media.MON was biosynthesized by two the same isolates of F. oxysporum in the amount up to 182.8 µg/g on rice and up to 743.3 µg/g on asparagus medium and one isolate (different on each medium) of F. proliferatum.

Key concepts: Asparagus, Fusarium proliferatum, Fusarium oxysporum, Biology, Fumonisin B1, Mycotoxin, Fumonisin, Fungi imperfecti

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