Recent Developments in Clean up Techniques of Pesticide Residue Analysis for Toxicology Study: A Critical Review

Clean up is one of the most important steps to remove co-extractives and undesired interfering substances from the sample before analysis in pesticide residue analysis. Some advanced, sophisticated and efficient methods of clean up have emerged out which is able to save time as well as amount of solvents. These are Solid Phase Extraction (SPE), Gel Permeation Chromatography (GPC) and Supercritical Fluid Chromatography (SFC). They are very much efficient for clean up and extraction of pesticide samples. SPE method is successfully used for clean up of pesticide residue from soil and water samples. GPC is now widely used in sample preparation in many residue laboratories and is effectively used for multi-residue analysis. SFC is by far the best method of clean up and extraction, though it involves high cost. Thus, various suitable clean up methods can free pesticide residues from extraneous co-extractive materials in an efficient manner and helps to prepare a good sample for further analysis.