2006•Journal of Clinical MicrobiologyOpen access

Two New Colorimetric Methods for Early Detection of Vancomycin and Oxacillin Resistance in Staphylococcus aureus

Ahmet Yılmaz Çoban, Bülent Bozdoğan, Çiğdem Çekiç Cihan, Ebru Cetinkaya, Kemal Bılgın, Özge Darka, Alper Akgüneş, Belma Durupınar, Peter C. Appelbaum

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Abstract

We developed two colorimetric methods for the detection of vancomycin- and oxacillin-resistant Staphylococcus aureus in </=6 h: (i) a nitrate reductase assay and (ii) a resazurin microplate method. MICs agreed with results obtained by CLSI methods for oxacillin. However, detection of vancomycin resistance required a larger inoculum. These methods may be recommended for the detection of vancomycin- and oxacillin-resistant S. aureus.

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Key concepts: Resazurin, Staphylococcus aureus, Vancomycin, Microbiology, Medicine, Chemistry, Biology, Bacteria